Hotspot

ycf34

Genome · 42104-42388 · 285 bp

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Pi 0.0363

MarkerSeek score 57.80

Primer available yes

Label ycf34

Feature Metadata

label_name: ycf34
feature_type: gene
region: Genome
length_bp: 285
variable_sites: 31
pi: 0.0363
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf34_p1	GCATCAGCATAGCAGGATGT	AACGTCAGAAACCTGGTGTC	50.0	50.0	58.1	58.1	1140 bp	0.307692	54.1
ycf34_p2	GCATCAGCATAGCAGGATGT	GACGCAACGGACTTAGAAGT	50.0	50.0	58.1	57.9	1168 bp	0.307692	53.9
ycf34_p3	GCATCAGCATAGCAGGATGT	CCTGTACGTTCTCAGGCATC	50.0	55.0	58.1	58.1	1591-1597 bp	0.307692	53.8
ycf34_p4	GCATCAGCATAGCAGGATGT	CTGTACGTTCTCAGGCATCC	50.0	55.0	58.1	58.1	1590-1596 bp	0.307692	53.8
ycf34_p5	GCATCAGCATAGCAGGATGT	GGTAGACGCAACGGACTTAG	50.0	55.0	58.1	58.1	1172 bp	0.307692	53.6