Hotspot

rpl9

Genome · 94221-94688 · 468 bp

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Pi 0.0312

MarkerSeek score 62.10

Primer available yes

Label rpl9

Feature Metadata

label_name: rpl9
feature_type: gene
region: Genome
length_bp: 468
variable_sites: 50
pi: 0.0312
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl9_p1	AGGACTTGAACCTACGACCA	AGCTGTTTTCCCCATTGAGG	50.0	50.0	58.0	58.1	1454-1455 bp	0.230769	49.9
rpl9_p2	CGAAGTCCCTAACCCATGTG	AGCTGTTTTCCCCATTGAGG	55.0	50.0	58.0	58.1	1872-1873 bp	0.230769	49.5
rpl9_p3	AACTTCTCCCCAGGTAGGAC	AGCTGTTTTCCCCATTGAGG	55.0	50.0	58.0	58.1	1469-1470 bp	0.230769	49.5
rpl9_p4	CTCCCCAGGTAGGACTTGAA	AGCTGTTTTCCCCATTGAGG	55.0	50.0	58.0	58.1	1464-1465 bp	0.230769	49.5
rpl9_p5	TTCTCCCCAGGTAGGACTTG	AGCTGTTTTCCCCATTGAGG	55.0	50.0	58.0	58.1	1466-1467 bp	0.230769	49.5