Hotspot

orf501

Genome · 61069-62481 · 1413 bp

Back to Sargassum

Pi 0.0519

MarkerSeek score 73.70

Primer available yes

Label orf501

Feature Metadata

label_name: orf501
feature_type: gene
region: Genome
length_bp: 1413
variable_sites: 215
pi: 0.0519
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 73.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
orf501_p1	TGTTGGTCCAATTTCAGGGG	GTTGGGTTGGCCGTAGTTAT	50.0	50.0	58.0	57.9	2428-2437 bp	0.769231	78.1
orf501_p2	TGTTGGTCCAATTTCAGGGG	AATGTCGTCGGGCTTCATTT	50.0	45.0	58.0	58.2	2334-2344 bp	0.769231	77.6
orf501_p3	ATCCCAAGGAAGCAAAGCAA	GTTGGGTTGGCCGTAGTTAT	45.0	50.0	58.0	57.9	2612-2621 bp	0.692308	74.9
orf501_p4	TCCCTATCCCAAGGAAGCAA	GTTGGGTTGGCCGTAGTTAT	50.0	50.0	58.0	57.9	2617-2626 bp	0.692308	74.9
orf501_p5	TTCCCTATCCCAAGGAAGCA	GTTGGGTTGGCCGTAGTTAT	50.0	50.0	58.0	57.9	2618-2627 bp	0.692308	74.9