Hotspot

ilvB

Genome · 81440-84115 · 2676 bp

Back to Sargassum

Pi 0.0282

MarkerSeek score 66.30

Primer available yes

Label ilvB

Feature Metadata

label_name: ilvB
feature_type: gene
region: Genome
length_bp: 2676
variable_sites: 229
pi: 0.0282
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ilvB_p1	AGGCTGGTAACTCAGAAGGA	TCCTAAAACAAATGGTTAGTGT	50.0	31.8	57.7	53.6	2834 bp	0.230769	10.0
ilvB_p2	AGGCTGGTAACTCAGAAGGA	ATCCTAAAACAAATGGTTAGTGT	50.0	30.4	57.7	54.2	2835 bp	0.230769	10.0
ilvB_p3	AGGCTGGTAACTCAGAAGGA	TCCTAAAACAAATGGTTAGTGTAGA	50.0	32.0	57.7	56.2	2834 bp	0.230769	10.0
ilvB_p4	AGGCTGGTAACTCAGAAGGA	CCTAAAACAAATGGTTAGTGTAGA	50.0	33.3	57.7	54.8	2833 bp	0.230769	10.0
ilvB_p5	AGGCTGGTAACTCAGAAGGA	TCCTAAAACAAATGGTTAGTGTAG	50.0	33.3	57.7	54.8	2834 bp	0.230769	10.0