Hotspot

trnN-GUU-rpl9

Genome · 93969-94220 · 252 bp

Back to Sargassum

Pi 0.0621

MarkerSeek score 74.50

Primer available yes

Label trnN-GUU-rpl9

Feature Metadata

label_name: trnN-GUU-rpl9
feature_type: igs
region: Genome
length_bp: 252
variable_sites: 45
pi: 0.0621
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 74.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-GUU-rpl9_p1	AGGACTTGAACCTACGACCA	TCGATAAGATAACTTGAACAGT	50.0	31.8	58.0	52.7	348-389 bp	1.0	51.0
trnN-GUU-rpl9_p2	AGGACTTGAACCTACGACCA	TTCGATAAGATAACTTGAACAGT	50.0	30.4	58.0	53.6	349-390 bp	1.0	51.0
trnN-GUU-rpl9_p3	AACTTCTCCCCAGGTAGGAC	TCGATAAGATAACTTGAACAGT	55.0	31.8	58.0	52.7	363-404 bp	1.0	50.9
trnN-GUU-rpl9_p4	CTCCCCAGGTAGGACTTGAA	TCGATAAGATAACTTGAACAGT	55.0	31.8	58.0	52.7	358-399 bp	1.0	50.9
trnN-GUU-rpl9_p5	TTCTCCCCAGGTAGGACTTG	TCGATAAGATAACTTGAACAGT	55.0	31.8	58.0	52.7	360-401 bp	1.0	50.9