Hotspot

trnC-GCA-rbcL

Genome · 42847-43108 · 262 bp

Back to Sargassum

Pi 0.0496

MarkerSeek score 74.10

Primer available yes

Label trnC-GCA-rbcL

Feature Metadata

label_name: trnC-GCA-rbcL
feature_type: igs
region: Genome
length_bp: 262
variable_sites: 38
pi: 0.0496
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 74.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-rbcL_p1	ATTTGCAATCCACGGCCTTA	TCGAGTTCTTTCCTGTACGT	45.0	45.0	58.2	56.3	317-336 bp	1.0	76.8
trnC-GCA-rbcL_p2	CAAATTAGGCGACACCCAGA	TCGAGTTCTTTCCTGTACGT	50.0	45.0	57.9	56.3	356-375 bp	0.538462	54.7
trnC-GCA-rbcL_p3	TCAAATTAGGCGACACCCAG	TCGAGTTCTTTCCTGTACGT	50.0	45.0	57.9	56.3	357-376 bp	0.538462	54.7
trnC-GCA-rbcL_p4	AGTCAAATTAGGCGACACCC	TCGAGTTCTTTCCTGTACGT	50.0	45.0	57.9	56.3	359-378 bp	0.538462	54.6
trnC-GCA-rbcL_p5	GTCAAATTAGGCGACACCCA	TCGAGTTCTTTCCTGTACGT	50.0	45.0	58.2	56.3	358-377 bp	0.538462	54.0