Hotspot

ycf54-secA

Genome · 13766-13877 · 112 bp

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Pi 0.0483

MarkerSeek score 73.20

Primer available yes

Label ycf54-secA

Feature Metadata

label_name: ycf54-secA
feature_type: igs
region: Genome
length_bp: 112
variable_sites: 18
pi: 0.0483
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 73.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf54-secA_p1	CGTGATGAGCCTGTTGAAGA	ACCCATCCATTCTGCATCAC	50.0	50.0	57.9	57.9	814-820 bp	0.692308	72.9
ycf54-secA_p2	GCTGTTCGAGCTGGTAGAAA	ACCCATCCATTCTGCATCAC	50.0	50.0	57.9	57.9	1168-1184 bp	0.615385	69.7
ycf54-secA_p3	AAGGCCTACTTAGCAGGACA	ACCCATCCATTCTGCATCAC	50.0	50.0	58.0	57.9	1100-1166 bp	0.538462	69.5
ycf54-secA_p4	AGGCCTACTTAGCAGGACAA	ACCCATCCATTCTGCATCAC	50.0	50.0	58.0	57.9	1099-1165 bp	0.538462	69.5
ycf54-secA_p5	AGGCCTACTTAGCAGGACAA	ATTAAAGGTGTCCGGGCTTC	50.0	50.0	58.0	57.9	1338-1389 bp	0.461538	65.0