Hotspot

psbI-trnG-GCC

Genome · 12138-12263 · 126 bp

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Pi 0.0868

MarkerSeek score 74.20

Primer available yes

Label psbI-trnG-GCC

Feature Metadata

label_name: psbI-trnG-GCC
feature_type: igs
region: Genome
length_bp: 126
variable_sites: 36
pi: 0.0868
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 74.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbI-trnG-GCC_p1	TCCAAGAGGAGTTCCACAGA	GTTGTTTTTGCACCAGCTGT	50.0	45.0	57.6	58.0	1247-1276 bp	1.0	86.8
psbI-trnG-GCC_p2	TCCAAGAGGAGTTCCACAGA	AATTCCTTGACCCGACCCTA	50.0	50.0	57.6	58.0	1524-1553 bp	1.0	86.2
psbI-trnG-GCC_p3	TCCAAGAGGAGTTCCACAGA	TTCCTTGACCCGACCCTATT	50.0	50.0	57.6	58.0	1522-1551 bp	1.0	86.2
psbI-trnG-GCC_p4	TCCAAGAGGAGTTCCACAGA	TTGGCAAGGTTACGCTCTAC	50.0	50.0	57.6	57.9	891-906 bp	1.0	85.8
psbI-trnG-GCC_p5	AGAGGAGTTCCACAGATCGA	GTTGTTTTTGCACCAGCTGT	50.0	45.0	57.5	58.0	1243-1272 bp	0.923077	82.1