Hotspot

psbX-ycf66

Genome · 6026-6240 · 215 bp

Back to Sargassum

Pi 0.0677

MarkerSeek score 75.50

Primer available yes

Label psbX-ycf66

Feature Metadata

label_name: psbX-ycf66
feature_type: igs
region: Genome
length_bp: 215
variable_sites: 44
pi: 0.0677
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 75.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbX-ycf66_p1	GCTATAACTCGTGTACCTCCA	GCCTAGAAGAAAGTTTGCTCC	47.6	47.6	56.7	56.9	280-299 bp	1.0	55.4
psbX-ycf66_p2	TGCTATAACTCGTGTACCTCC	GCCTAGAAGAAAGTTTGCTCC	47.6	47.6	56.7	56.9	281-300 bp	1.0	55.4
psbX-ycf66_p3	TGCTATAACTCGTGTACCTCCA	GCCTAGAAGAAAGTTTGCTCC	45.5	47.6	58.4	56.9	281-300 bp	1.0	55.1
psbX-ycf66_p4	GCTATAACTCGTGTACCTCCA	AGCCTAGAAGAAAGTTTGCTCC	47.6	45.5	56.7	58.3	281-300 bp	1.0	53.7
psbX-ycf66_p5	TGCTATAACTCGTGTACCTCC	AGCCTAGAAGAAAGTTTGCTCC	47.6	45.5	56.7	58.3	282-301 bp	1.0	53.7