Hotspot

ycf1

SSC · 122929-128379 · 5451 bp

Back to Sarcopyramis

Pi 0.00917

MarkerSeek score 45.20

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5451
variable_sites: 50
pi: 0.00917
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 45.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	CTGTTTCGCCATTTGTGTGG	CCTTTTGGTTCTCCCCGAAA	50.0	50.0	58.2	58.0	974 bp	1.0	79.6
ycf1_p2	TTCGCCATTTGTGTGGTACT	CCTTTTGGTTCTCCCCGAAA	45.0	50.0	57.7	58.0	970 bp	1.0	79.4
ycf1_p3	TGGCGAAGGAATTCGATTCA	CCTTTTGGTTCTCCCCGAAA	45.0	50.0	57.6	58.0	1093 bp	1.0	78.4
ycf1_p4	CTGTTTCGCCATTTGTGTGG	CCTTCCTTTTGGTTCTCCCC	50.0	55.0	58.2	58.1	978 bp	0.5	57.6
ycf1_p5	TTCGCCATTTGTGTGGTACT	CCTTCCTTTTGGTTCTCCCC	45.0	55.0	57.7	58.1	974 bp	0.5	57.3