Hotspot

rps16

LSC · 4635-5670 · 1036 bp

Back to Sarcopyramis

Pi 0.0145

MarkerSeek score 57.40

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: LSC
length_bp: 1036
variable_sites: 15
pi: 0.0145
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	AGGGAGAGGGGGATCTACTA	ATACCAGTTAAAACCCGGGG	55.0	50.0	57.4	57.2	1131-1133 bp	1.0	70.3
rps16_p2	AGGGAGAGGGGGATCTACTA	ACATACCAGTTAAAACCCGGG	55.0	47.6	57.4	57.9	1133-1135 bp	1.0	68.2
rps16_p3	GAGGGAGAGGGGGATCTACT	ATACCAGTTAAAACCCGGGG	60.0	50.0	58.8	57.2	1132-1134 bp	1.0	68.0
rps16_p4	GGGAGAGGGGGATCTACTAG	ATACCAGTTAAAACCCGGGG	60.0	50.0	57.1	57.2	1130-1132 bp	1.0	67.4
rps16_p5	AGGGAGAGGGGGATCTACTA	TACCAGTTAAAACCCGGGGG	55.0	55.0	57.4	59.3	1130-1132 bp	1.0	66.4