Hotspot

rps15

SSC · 122337-122600 · 264 bp

Back to Sarcopyramis

Pi 0.0189

MarkerSeek score 48.80

Primer available yes

Label rps15

Feature Metadata

label_name: rps15
feature_type: gene
region: SSC
length_bp: 264
variable_sites: 5
pi: 0.0189
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15_p1	AACACCATGCATTGATGGGT	ATGGAAGCCGCTTTAGTCTG	45.0	50.0	58.1	58.0	844-852 bp	1.0	80.7
rps15_p2	TGCATTGATGGGTGATGAGG	ATGGAAGCCGCTTTAGTCTG	50.0	50.0	57.9	58.0	837-845 bp	1.0	80.7
rps15_p3	GCATTGATGGGTGATGAGGT	ATGGAAGCCGCTTTAGTCTG	50.0	50.0	57.9	58.0	836-844 bp	1.0	80.7
rps15_p4	TAACTGTTCGGTTTTCGGCA	ATGGAAGCCGCTTTAGTCTG	45.0	50.0	58.0	58.0	950-958 bp	1.0	80.6
rps15_p5	AAGGGAGTTTGTGCACCAAT	ATGGAAGCCGCTTTAGTCTG	45.0	50.0	57.9	58.0	1161-1169 bp	1.0	80.2