Hotspot

trnN-GUU-trnR-ACG

IRa · 128753-129347 · 595 bp

Back to Sarcopyramis

Pi 0.00349
MarkerSeek score 64.20
Primer available yes
Label trnN-GUU-trnR-ACG

Feature Metadata

label_name
trnN-GUU-trnR-ACG
feature_type
igs
region
IRa
length_bp
595
variable_sites
2
pi
0.00349
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.96
markerseek_score
64.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnN-GUU-trnR-ACG_p1 CCGTTGTTCCTCAGTAGCTC CAGAGGATTAGAGCACGTGG 55.0 55.0 58.0 57.8 714-736 bp 1.0 80.9
trnN-GUU-trnR-ACG_p2 CCGTTGTTCCTCAGTAGCTC GGGTACTCCCGAGAACAGAT 55.0 55.0 58.0 58.2 763-785 bp 1.0 80.7
trnN-GUU-trnR-ACG_p3 CCGTTGTTCCTCAGTAGCTC GAACAGATCCAGTGGAGACG 55.0 55.0 58.0 57.7 751-773 bp 1.0 80.2
trnN-GUU-trnR-ACG_p4 GCTGTTAACTGACTGGTCGT CAGAGGATTAGAGCACGTGG 50.0 55.0 57.8 57.8 678-700 bp 1.0 79.9
trnN-GUU-trnR-ACG_p5 GCTGTTAACTGACTGGTCGT GGGTACTCCCGAGAACAGAT 50.0 55.0 57.8 58.2 727-749 bp 1.0 79.6