Hotspot

ndhE-ndhG

SSC · 117148-117377 · 230 bp

Back to Sarcopyramis

Pi 0.0448

MarkerSeek score 66.40

Primer available yes

Label ndhE-ndhG

Feature Metadata

label_name: ndhE-ndhG
feature_type: igs
region: SSC
length_bp: 230
variable_sites: 10
pi: 0.0448
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 66.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhE-ndhG_p1	ACTCCTGGGTTGGCTAAGAT	TTTGGACTGTTGGGAATGGG	50.0	50.0	58.0	58.0	901-914 bp	1.0	81.9
ndhE-ndhG_p2	GCCTTACATCCATCCCAAGG	TTTGGACTGTTGGGAATGGG	55.0	50.0	58.0	58.0	1137-1150 bp	1.0	81.6
ndhE-ndhG_p3	CGTGGAGCAGAAGCAATTTG	TTTGGACTGTTGGGAATGGG	50.0	50.0	58.0	58.0	1161-1174 bp	1.0	81.4
ndhE-ndhG_p4	AATACATCGGTTGGACAGGC	TTTGGACTGTTGGGAATGGG	50.0	50.0	58.0	58.0	1107-1120 bp	1.0	81.4
ndhE-ndhG_p5	TCGTGGAGCAGAAGCAATTT	TTTGGACTGTTGGGAATGGG	45.0	50.0	58.1	58.0	1162-1175 bp	1.0	80.8