Hotspot

ndhF-rpl32

SSC · 112559-112887 · 329 bp

Back to Sarcopyramis

Pi 0.0304

MarkerSeek score 57.70

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 329
variable_sites: 10
pi: 0.0304
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	TGGGAAGTGGAATGAGAGGT	AGCGTGTCTACCGATTTCAC	50.0	50.0	58.0	58.0	851-872 bp	1.0	81.2
ndhF-rpl32_p2	TGGGAAGTGGAATGAGAGGT	CAGCGTGTCTACCGATTTCA	50.0	50.0	58.0	58.0	852-873 bp	1.0	81.1
ndhF-rpl32_p3	AGCAAAGGACCACATACGTC	AGCGTGTCTACCGATTTCAC	50.0	50.0	57.9	58.0	928-949 bp	1.0	80.6
ndhF-rpl32_p4	AGCAAAGGACCACATACGTC	CAGCGTGTCTACCGATTTCA	50.0	50.0	57.9	58.0	929-950 bp	1.0	80.6
ndhF-rpl32_p5	CGCCTACACGATTCGTTACA	AGCGTGTCTACCGATTTCAC	50.0	50.0	58.1	58.0	1355-1376 bp	1.0	80.2