Hotspot

rps4-trnT-UGU

LSC · 46184-46574 · 391 bp

Back to Sarcopyramis

Pi 0.0256

MarkerSeek score 65.20

Primer available yes

Label rps4-trnT-UGU

Feature Metadata

label_name: rps4-trnT-UGU
feature_type: igs
region: LSC
length_bp: 391
variable_sites: 10
pi: 0.0256
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps4-trnT-UGU_p1	TAGACCTGGTAAAGCCCCAA	CTCAGAGGTTAGAGCATCGC	50.0	55.0	58.0	57.9	517-524 bp	1.0	80.2
rps4-trnT-UGU_p2	TTAGACCTGGTAAAGCCCCA	CTCAGAGGTTAGAGCATCGC	50.0	55.0	58.0	57.9	518-525 bp	1.0	80.2
rps4-trnT-UGU_p3	TGAAACGAGGCCCCCTATAA	CTCAGAGGTTAGAGCATCGC	50.0	55.0	58.1	57.9	482-489 bp	1.0	79.8
rps4-trnT-UGU_p4	TTGAAACGAGGCCCCCTATA	CTCAGAGGTTAGAGCATCGC	50.0	55.0	58.1	57.9	483-490 bp	1.0	79.8
rps4-trnT-UGU_p5	TAGACCTGGTAAAGCCCCAA	ATGGTCATCGGTTCGATTCC	50.0	50.0	58.0	57.8	485-492 bp	1.0	79.5