Hotspot

trnT-GGU-psbD

LSC · 31924-33084 · 1161 bp

Back to Sarcopyramis

Pi 0.013
MarkerSeek score 66.80
Primer available yes
Label trnT-GGU-psbD

Feature Metadata

label_name
trnT-GGU-psbD
feature_type
igs
region
LSC
length_bp
1161
variable_sites
15
pi
0.013
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
66.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-GGU-psbD_p1 AGGAGCCCCTTTAACTCAGT GAAACGGTCCCTACGTAACC 50.0 55.0 58.0 58.0 1321-1331 bp 1.0 80.9
trnT-GGU-psbD_p2 GTGGTAGAGTAACGCCATGG GAAACGGTCCCTACGTAACC 55.0 55.0 58.1 58.0 1303-1313 bp 1.0 80.8
trnT-GGU-psbD_p3 TCAGTGGTAGAGTAACGCCA GAAACGGTCCCTACGTAACC 50.0 55.0 58.1 58.0 1306-1316 bp 1.0 80.6
trnT-GGU-psbD_p4 GAGCCCCTTTAACTCAGTGG GAAACGGTCCCTACGTAACC 55.0 55.0 57.9 58.0 1319-1329 bp 1.0 80.4
trnT-GGU-psbD_p5 GGAGCCCCTTTAACTCAGTG GAAACGGTCCCTACGTAACC 55.0 55.0 57.9 58.0 1320-1330 bp 1.0 80.4