Hotspot

trnC-GCA-petN

LSC · 28189-29008 · 820 bp

Back to Sarcopyramis

Pi 0.0134

MarkerSeek score 66.50

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 820
variable_sites: 11
pi: 0.0134
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	TTTCCCCAGTTCAAATCCGG	TTAAAGCAGCCCAAGCAAGA	50.0	45.0	58.1	58.0	883-890 bp	1.0	80.3
trnC-GCA-petN_p2	TTTCCCCAGTTCAAATCCGG	ACAACCATTAAAGCAGCCCA	50.0	45.0	58.1	58.0	890-897 bp	1.0	80.3
trnC-GCA-petN_p3	TTTCCCCAGTTCAAATCCGG	CCTTTAGAGTCCACTTCGCC	50.0	55.0	58.1	58.0	942-949 bp	1.0	80.2
trnC-GCA-petN_p4	TTTCCCCAGTTCAAATCCGG	CTTTAGAGTCCACTTCGCCC	50.0	55.0	58.1	58.0	941-948 bp	1.0	80.2
trnC-GCA-petN_p5	TTTCCCCAGTTCAAATCCGG	CAGCCCAAGCAAGACTTACT	50.0	50.0	58.1	57.8	877-884 bp	1.0	78.8