Hotspot

trnQ-UUG-psbK

LSC · 6689-7010 · 322 bp

Back to Sarcopyramis

Pi 0.019
MarkerSeek score 52.50
Primer available yes
Label trnQ-UUG-psbK

Feature Metadata

label_name
trnQ-UUG-psbK
feature_type
igs
region
LSC
length_bp
322
variable_sites
6
pi
0.019
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
52.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnQ-UUG-psbK_p1 CCTTACCACTTGGCCACG AGGGTGAAATGAAAACAGAAGT 61.1 36.4 58.0 56.1 392-394 bp 1.0 42.4
trnQ-UUG-psbK_p2 CCTTACCACTTGGCCACG AAGGGTGAAATGAAAACAGAAGT 61.1 34.8 58.0 56.8 393-395 bp 1.0 42.4
trnQ-UUG-psbK_p3 GCCTTACCACTTGGCCAC AGGGTGAAATGAAAACAGAAGT 61.1 36.4 58.3 56.1 393-395 bp 1.0 42.4
trnQ-UUG-psbK_p4 CCTTACCACTTGGCCACG GGGTGAAATGAAAACAGAAGT 61.1 38.1 58.0 54.6 391-393 bp 1.0 42.4
trnQ-UUG-psbK_p5 GCCTTACCACTTGGCCAC AAGGGTGAAATGAAAACAGAAGT 61.1 34.8 58.3 56.8 394-396 bp 1.0 42.4