Hotspot

rps16-trnQ-UUG

LSC · 5671-6616 · 946 bp

Back to Sarcopyramis

Pi 0.0163
MarkerSeek score 57.90
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
946
variable_sites
15
pi
0.0163
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.97
markerseek_score
57.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 CGTTGCTTTCTACCACATCG GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.2 58.1 1011-1013 bp 1.0 76.3
rps16-trnQ-UUG_p2 GCACGTTGCTTTCTACCACA GAGGTTCGAATCCTTCCGTC 50.0 55.0 59.1 58.1 1014-1016 bp 1.0 74.1
rps16-trnQ-UUG_p3 TAAGAATCCACGGACGATCC GAGGTTCGAATCCTTCCGTC 50.0 55.0 56.8 58.1 1047-1049 bp 1.0 73.1
rps16-trnQ-UUG_p4 CGTTGCTTTCTACCACATCG TTCGGAGGTTCGAATCCTTC 50.0 50.0 57.2 57.3 1015-1017 bp 1.0 71.5
rps16-trnQ-UUG_p5 CACGTTGCTTTCTACCACATC GAGGTTCGAATCCTTCCGTC 47.6 55.0 57.5 58.1 1013-1015 bp 1.0 70.9