Hotspot

ycf1

SSC · 112929-118586 · 5658 bp

Back to Sarcandra

Pi 0.334

MarkerSeek score 74.70

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5658
variable_sites: 1616
pi: 0.334
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.85
markerseek_score: 74.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	AAGAGGGATCCACCGAAGAA	CGGTTCATTCCCCATCCAAT	50.0	50.0	58.0	57.9	1508 bp	0.5	59.0
ycf1_p2	TTGCACACATGTCCAAGTGA	CGGTTCATTCCCCATCCAAT	45.0	50.0	58.0	57.9	1170 bp	0.5	59.0
ycf1_p3	AAGAGGGATCCACCGAAGAA	CACCTCTTCTGCTCGATCAC	50.0	55.0	58.0	58.1	1289 bp	0.5	59.0
ycf1_p4	AAGAGGGATCCACCGAAGAA	CCGTTCATCCTCGTCTTGTT	50.0	50.0	58.0	57.9	1592 bp	0.5	59.0
ycf1_p5	TTGCACACATGTCCAAGTGA	CACCTCTTCTGCTCGATCAC	45.0	55.0	58.0	58.1	951 bp	0.5	59.0