Hotspot

ndhH

SSC · 119296-120483 · 1188 bp

Back to Sarcandra

Pi 0.495

MarkerSeek score 79.60

Primer available yes

Label ndhH

Feature Metadata

label_name: ndhH
feature_type: gene
region: SSC
length_bp: 1188
variable_sites: 471
pi: 0.495
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.80
markerseek_score: 79.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhH_p1	GAAAACGTCAACGACTGCTG	TACGTTGTTGTATCCCTGCG	50.0	50.0	58.0	58.0	1578 bp	0.5	60.0
ndhH_p2	CCAGATCGAAATGACTGGCA	TACGTTGTTGTATCCCTGCG	50.0	50.0	58.0	58.0	1824 bp	0.5	59.8
ndhH_p3	ACCAGATCGAAATGACTGGC	TACGTTGTTGTATCCCTGCG	50.0	50.0	58.0	58.0	1825 bp	0.5	59.8
ndhH_p4	GAAAACGTCAACGACTGCTG	TGTTCCAACGAATCACACGT	50.0	45.0	58.0	58.1	1965 bp	0.5	59.5
ndhH_p5	CCAGATCGAAATGACTGGCA	TGTTCCAACGAATCACACGT	50.0	45.0	58.0	58.1	2211 bp	0.5	59.3