Hotspot

ndhD

SSC · 125344-126846 · 1503 bp

Back to Sarcandra

Pi 0.482

MarkerSeek score 80.80

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 1503
variable_sites: 616
pi: 0.482
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.85
markerseek_score: 80.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	GCTATTGGGCCAGCTATTGT	AGGTGCAAATTCGGCAATTG	50.0	45.0	58.0	57.9	2585 bp	0.5	59.2
ndhD_p2	GCTATTGGGCCAGCTATTGT	CAAGGTGCAAATTCGGCAAT	50.0	45.0	58.0	57.9	2587 bp	0.5	59.2
ndhD_p3	GCTATTGGGCCAGCTATTGT	GAGCGGTATGGGCTAATGAG	50.0	55.0	58.0	57.9	2709 bp	0.5	59.2
ndhD_p4	TCGAATGAGACAACTCGCAG	AGGTGCAAATTCGGCAATTG	50.0	45.0	58.0	57.9	2051 bp	0.5	59.2
ndhD_p5	TCGAATGAGACAACTCGCAG	CAAGGTGCAAATTCGGCAAT	50.0	45.0	58.0	57.9	2053 bp	0.5	59.2