Hotspot

ccsA

SSC · 127105-128067 · 963 bp

Back to Sarcandra

Pi 0.48

MarkerSeek score 81.80

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: SSC
length_bp: 963
variable_sites: 408
pi: 0.48
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.88
markerseek_score: 81.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	GGATTCTGGACCACGAGAAC	GTGAAATCGGTAGACACGCT	55.0	50.0	58.0	58.0	1529 bp	0.5	59.8
ccsA_p2	GGATTCTGGACCACGAGAAC	TGAAATCGGTAGACACGCTG	55.0	50.0	58.0	58.0	1528 bp	0.5	59.8
ccsA_p3	TTCTCGATGGCTTCTCTTGC	GTGAAATCGGTAGACACGCT	50.0	50.0	58.0	58.0	1753 bp	0.5	59.8
ccsA_p4	TTCTCGATGGCTTCTCTTGC	TGAAATCGGTAGACACGCTG	50.0	50.0	58.0	58.0	1752 bp	0.5	59.8
ccsA_p5	TCAGTTGACAAGGTGGAAGC	GTGAAATCGGTAGACACGCT	50.0	50.0	58.0	58.0	1438 bp	0.5	59.7