Hotspot

rpl32-ndhF

SSC · 129335-130447 · 1113 bp

Back to Sarcandra

Pi 0.438

MarkerSeek score 82.30

Primer available yes

Label rpl32-ndhF

Feature Metadata

label_name: rpl32-ndhF
feature_type: igs
region: SSC
length_bp: 1113
variable_sites: 440
pi: 0.438
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 82.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-ndhF_p1	CATCGGGTCATGCTCATTCA	GGAACAAGCAGAAGTCCCAA	50.0	50.0	58.0	58.0	1405 bp	0.5	59.5
rpl32-ndhF_p2	CATCGGGTCATGCTCATTCA	CGAAAGGTATGACCCATGCA	50.0	50.0	58.0	58.0	1356 bp	0.5	59.4
rpl32-ndhF_p3	CATCGGGTCATGCTCATTCA	GCCCACATACGACGAAGATT	50.0	50.0	58.0	58.1	1438 bp	0.5	59.1
rpl32-ndhF_p4	CATCGGGTCATGCTCATTCA	ACGAACACATTCCCACTAGC	50.0	50.0	58.0	57.9	1791 bp	0.5	58.9
rpl32-ndhF_p5	CATCGGGTCATGCTCATTCA	AGGAAAAGCCCACATACGAC	50.0	50.0	58.0	57.9	1445 bp	0.5	58.8