Hotspot

ndhG-ndhE

SSC · 124166-124424 · 259 bp

Back to Sarcandra

Pi 0.473

MarkerSeek score 77.30

Primer available yes

Label ndhG-ndhE

Feature Metadata

label_name: ndhG-ndhE
feature_type: igs
region: SSC
length_bp: 259
variable_sites: 116
pi: 0.473
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 77.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG-ndhE_p1	ACGTGGGAGCCATAAATGTC	ACAATAGCTGGCCCAATAGC	50.0	50.0	58.0	58.0	833 bp	0.5	59.6
ndhG-ndhE_p2	GCCCGGTAAGTAAGGTCATG	ACAATAGCTGGCCCAATAGC	55.0	50.0	58.1	58.0	1086 bp	0.5	59.5
ndhG-ndhE_p3	ACGTGGGAGCCATAAATGTC	ACCATGTTTCGGCTTGTGAT	50.0	45.0	58.0	58.1	674 bp	0.5	59.0
ndhG-ndhE_p4	GGATACAGAAGCGGGATTGG	ACAATAGCTGGCCCAATAGC	55.0	50.0	58.1	58.0	1209 bp	0.5	59.0
ndhG-ndhE_p5	ACGTGGGAGCCATAAATGTC	GTCCTCTGTTCTTGGAGCAG	50.0	55.0	58.0	57.9	1281 bp	0.5	59.0