Hotspot

ndhA

SSC · 123184-125442 · 2259 bp

Back to Saracha

Pi 0.00444

MarkerSeek score 60.10

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2259
variable_sites: 10
pi: 0.00444
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	TGTTTGAGACAAGTCGTGAA	TGGGAGAAGTTGATCGTTGA	40.0	45.0	55.2	56.5	2311-2319 bp	1.0	46.1
ndhA_p2	TTGTTTGAGACAAGTCGTGA	TGGGAGAAGTTGATCGTTGA	40.0	45.0	55.2	56.5	2312-2320 bp	1.0	46.1
ndhA_p3	TTGTTTGAGACAAGTCGTGAA	TGGGAGAAGTTGATCGTTGA	38.1	45.0	56.0	56.5	2312-2320 bp	1.0	44.4
ndhA_p4	TGTTTGAGACAAGTCGTGAATC	TGGGAGAAGTTGATCGTTGA	40.9	45.0	56.9	56.5	2311-2319 bp	1.0	44.1
ndhA_p5	TGTTTGAGACAAGTCGTGAA	TGGGAGAAGTTGATCGTTGAA	40.0	42.9	55.2	57.2	2311-2319 bp	1.0	44.1