Hotspot

trnI-CAU-rpl23

IRa · 155497-155660 · 164 bp

Back to Saracha

Pi 0.0312

MarkerSeek score 57.90

Primer available yes

Label trnI-CAU-rpl23

Feature Metadata

label_name: trnI-CAU-rpl23
feature_type: igs
region: IRa
length_bp: 164
variable_sites: 5
pi: 0.0312
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 57.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnI-CAU-rpl23_p1	GAATGGTTAAAGCGCCCAAC	AGAAGAGTTCGACCCAATGC	50.0	50.0	58.0	57.9	350-354 bp	1.0	81.4
trnI-CAU-rpl23_p2	GAATGGTTAAAGCGCCCAAC	GAAGAGTTCGACCCAATGCT	50.0	50.0	58.0	57.9	349-353 bp	1.0	81.4
trnI-CAU-rpl23_p3	GAATGGTTAAAGCGCCCAAC	CTCTTTCCCGGAAGTCGATG	50.0	55.0	58.0	58.1	399-403 bp	1.0	81.3
trnI-CAU-rpl23_p4	GAATGGTTAAAGCGCCCAAC	ACTGTCTACGGTTCCATTGC	50.0	50.0	58.0	57.9	583-587 bp	1.0	80.5
trnI-CAU-rpl23_p5	GAATGGTTAAAGCGCCCAAC	AAACCGCACTCAAAGGTAGG	50.0	50.0	58.0	58.1	923-926 bp	1.0	79.9