Hotspot

ccsA-ndhD

SSC · 118786-119014 · 229 bp

Back to Saracha

Pi 0.0218

MarkerSeek score 52.50

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 229
variable_sites: 5
pi: 0.0218
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	ACCCAAGGCTTTGCTACTTC	TAGCTTTTCGATGGCTTCCC	50.0	50.0	58.1	58.0	1214 bp	1.0	79.6
ccsA-ndhD_p2	CCCAAGGCTTTGCTACTTCA	TAGCTTTTCGATGGCTTCCC	50.0	50.0	58.1	58.0	1213 bp	1.0	79.6
ccsA-ndhD_p3	ACCCAAGGCTTTGCTACTTC	TATCTTGACGAAATGGGCGG	50.0	50.0	58.1	58.1	1273 bp	1.0	79.4
ccsA-ndhD_p4	ACCCAAGGCTTTGCTACTTC	TGACGAAATGGGCGGAATAG	50.0	50.0	58.1	58.1	1268 bp	1.0	79.4
ccsA-ndhD_p5	CCCAAGGCTTTGCTACTTCA	TGACGAAATGGGCGGAATAG	50.0	50.0	58.1	58.1	1267 bp	1.0	79.4