Hotspot

trnR-ACG-trnN-GUU

IRb · 111596-112179 · 584 bp

Back to Saracha

Pi 0.00862

MarkerSeek score 64.00

Primer available yes

Label trnR-ACG-trnN-GUU

Feature Metadata

label_name: trnR-ACG-trnN-GUU
feature_type: igs
region: IRb
length_bp: 584
variable_sites: 5
pi: 0.00862
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 64.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-ACG-trnN-GUU_p1	CAGAGGATTAGAGCACGTGG	TGGTCGTAGGTTCGAATCCT	55.0	50.0	57.8	58.2	673-677 bp	1.0	78.1
trnR-ACG-trnN-GUU_p2	CAGAGGATTAGAGCACGTGG	TGGGAACGGGAATTGAACTC	55.0	50.0	57.8	57.8	758-762 bp	1.0	77.7
trnR-ACG-trnN-GUU_p3	GAACAGATCCAGTGGAGACG	TGGTCGTAGGTTCGAATCCT	55.0	50.0	57.7	58.2	710-714 bp	1.0	77.5
trnR-ACG-trnN-GUU_p4	CAGAGGATTAGAGCACGTGG	CGATTGGTCGTAGGTTCGAA	55.0	50.0	57.8	57.7	677-681 bp	1.0	77.2
trnR-ACG-trnN-GUU_p5	GAACAGATCCAGTGGAGACG	TGGGAACGGGAATTGAACTC	55.0	50.0	57.7	57.8	795-799 bp	1.0	77.1