Hotspot

petG-trnW-CCA

LSC · 70061-70191 · 131 bp

Back to Saracha

Pi 0.0153

MarkerSeek score 61.30

Primer available yes

Label petG-trnW-CCA

Feature Metadata

label_name: petG-trnW-CCA
feature_type: igs
region: LSC
length_bp: 131
variable_sites: 2
pi: 0.0153
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petG-trnW-CCA_p1	ATAGGCGTGGTGATCAGTTG	AGCGCTCTTAGTTCAGTTCG	50.0	50.0	57.7	58.0	235 bp	1.0	78.0
petG-trnW-CCA_p2	ATAGGCGTGGTGATCAGTTG	CAAAACCCGATGTCGTAGGT	50.0	50.0	57.7	57.9	199 bp	1.0	77.6
petG-trnW-CCA_p3	GTGGTGATCAGTTGGACCTT	AGCGCTCTTAGTTCAGTTCG	50.0	50.0	57.4	58.0	229 bp	1.0	76.0
petG-trnW-CCA_p4	GTGGTGATCAGTTGGACCTT	CAAAACCCGATGTCGTAGGT	50.0	50.0	57.4	57.9	193 bp	1.0	75.6
petG-trnW-CCA_p5	ATAGGCGTGGTGATCAGTTG	CGCTCTTAGTTCAGTTCGGT	50.0	50.0	57.7	57.7	233 bp	1.0	75.4