Hotspot

accD-psaI

LSC · 62191-62937 · 747 bp

Back to Saracha

Pi 0.0027

MarkerSeek score 61.10

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 747
variable_sites: 2
pi: 0.0027
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 61.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	TCCAGAAGGGTTTATTCGACC	AAGAAGCCATTGCAAATGCC	47.6	45.0	57.4	57.9	942-951 bp	1.0	66.9
accD-psaI_p2	AGTTATTTAAGCTCCACGCCT	AAGAAGCCATTGCAAATGCC	42.9	45.0	57.4	57.9	882-891 bp	1.0	66.9
accD-psaI_p3	CCAGAAGGGTTTATTCGACCT	AAGAAGCCATTGCAAATGCC	47.6	45.0	57.4	57.9	941-950 bp	1.0	66.8
accD-psaI_p4	TCGACCTAATTGTACCACGT	AAGAAGCCATTGCAAATGCC	45.0	45.0	56.3	57.9	927-936 bp	1.0	66.3
accD-psaI_p5	TCCAGAAGGGTTTATTCGACC	TGCAAATGCCGGAAAGACTA	47.6	45.0	57.4	57.8	932-941 bp	1.0	66.2