Hotspot

psaA-ycf3

LSC · 44113-44866 · 754 bp

Back to Saracha

Pi 0.00538
MarkerSeek score 63.00
Primer available yes
Label psaA-ycf3

Feature Metadata

label_name
psaA-ycf3
feature_type
igs
region
LSC
length_bp
754
variable_sites
4
pi
0.00538
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
63.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psaA-ycf3_p1 GCCCATTCCTCGAAAGAAGT ATCAAGCCGCTGAGTATTGG 50.0 50.0 57.9 58.0 921-932 bp 1.0 80.0
psaA-ycf3_p2 GCCCATTCCTCGAAAGAAGT GGAAACAGGCTATAGCGCTT 50.0 50.0 57.9 58.0 903-914 bp 1.0 80.0
psaA-ycf3_p3 GCCCATTCCTCGAAAGAAGT CAGAGGCTTGGTTCGATCAA 50.0 50.0 57.9 57.9 936-947 bp 1.0 79.6
psaA-ycf3_p4 GCCCATTCCTCGAAAGAAGT CGATCAAGCCGCTGAGTATT 50.0 50.0 57.9 58.1 923-934 bp 1.0 79.2
psaA-ycf3_p5 GCCCATTCCTCGAAAGAAGT AGCCGCTGAGTATTGGAAAC 50.0 50.0 57.9 58.3 917-928 bp 1.0 78.3