Hotspot

petN-psbM

LSC · 30307-31409 · 1103 bp

Back to Saracha

Pi 0.00272

MarkerSeek score 60.40

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 1103
variable_sites: 3
pi: 0.00272
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	TCTTGCTTGGGCTGCTTTAA	AGTTCCTACCGCTTTTCTACT	45.0	42.9	58.0	56.4	1236-1237 bp	1.0	59.3
petN-psbM_p2	AGTAAGTCTTGCTTGGGCTG	AGTTCCTACCGCTTTTCTACT	50.0	42.9	57.8	56.4	1242-1243 bp	1.0	57.8
petN-psbM_p3	GGGGAAGGAGTGGACTCTAG	AGTTCCTACCGCTTTTCTACT	60.0	42.9	58.2	56.4	1180-1181 bp	1.0	57.6
petN-psbM_p4	TCTTGCTTGGGCTGCTTTAA	AGTTCCTACCGCTTTTCTACTT	45.0	40.9	58.0	57.1	1236-1237 bp	1.0	57.1
petN-psbM_p5	TCTTGCTTGGGCTGCTTTAA	TCTAGTTCCTACCGCTTTTCT	45.0	42.9	58.0	56.1	1239-1240 bp	1.0	57.1