Hotspot

rpoB-trnC-GCA

LSC · 28119-29472 · 1354 bp

Back to Saracha

Pi 0.00369

MarkerSeek score 60.90

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1354
variable_sites: 5
pi: 0.00369
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	CTCATTTCCATCCCCGAGC	CCGGATTTGAACTGGGGAAA	57.9	50.0	58.0	58.1	1438 bp	1.0	71.5
rpoB-trnC-GCA_p2	ATTCCCTCATTTCCATCCCC	CCGGATTTGAACTGGGGAAA	50.0	50.0	56.9	58.1	1443 bp	1.0	71.0
rpoB-trnC-GCA_p3	TCATTTCCATCCCCGAGCAT	CCGGATTTGAACTGGGGAAA	50.0	50.0	59.2	58.1	1437 bp	1.0	70.4
rpoB-trnC-GCA_p4	CTCATTTCCATCCCCGAGC	AGTCCTCTGCCTTACCACTC	57.9	55.0	58.0	58.4	1407 bp	1.0	68.7
rpoB-trnC-GCA_p5	CATTTCCATCCCCGAGCAT	CCGGATTTGAACTGGGGAAA	52.6	50.0	57.6	58.1	1436 bp	1.0	68.2