Hotspot

psbI-trnS-GCU

LSC · 9215-9336 · 122 bp

Back to Saracha

Pi 0.0246

MarkerSeek score 66.60

Primer available yes

Label psbI-trnS-GCU

Feature Metadata

label_name: psbI-trnS-GCU
feature_type: igs
region: LSC
length_bp: 122
variable_sites: 3
pi: 0.0246
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbI-trnS-GCU_p1	ATGATCCCGGACGTAATCCT	TAAAGCGGCGGATTGCTAAT	50.0	45.0	58.0	58.0	225 bp	1.0	80.7
psbI-trnS-GCU_p2	ATGATCCCGGACGTAATCCT	ATTGGGAGAGATGGCTGAGT	50.0	50.0	58.0	58.1	249 bp	1.0	80.0
psbI-trnS-GCU_p3	ATGATCCCGGACGTAATCCT	CGAGTTAATCGTACCGAGGG	50.0	55.0	58.0	57.6	197 bp	1.0	78.0
psbI-trnS-GCU_p4	ATGATCCCGGACGTAATCCT	ATGGCTGAGTGGACTAAAGC	50.0	50.0	58.0	57.6	239 bp	1.0	77.6
psbI-trnS-GCU_p5	ATGATCCCGGACGTAATCCT	GAGAGATGGCTGAGTGGACT	50.0	55.0	58.0	58.5	244 bp	1.0	76.8