Hotspot

trnK-UUU-rps16

LSC · 5072-5767 · 696 bp

Back to Saracha

Pi 0.00718
MarkerSeek score 48.90
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
696
variable_sites
5
pi
0.00718
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
48.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 CCCTTTCAGGATCAGTCGTG AAAAGGGTATGTTGCTGCCA 55.0 45.0 58.0 58.0 1529-1542 bp 1.0 80.9
trnK-UUU-rps16_p2 CCTTTCAGGATCAGTCGTGG AAAAGGGTATGTTGCTGCCA 55.0 45.0 58.0 58.0 1528-1541 bp 1.0 80.9
trnK-UUU-rps16_p3 TCCCTTTCAGGATCAGTCGT AAAAGGGTATGTTGCTGCCA 50.0 45.0 58.1 58.0 1530-1543 bp 1.0 80.5
trnK-UUU-rps16_p4 TCAGGATCAGTCGTGGTCTT AAAAGGGTATGTTGCTGCCA 50.0 45.0 58.1 58.0 1524-1537 bp 1.0 80.4
trnK-UUU-rps16_p5 TTCAGGATCAGTCGTGGTCT AAAAGGGTATGTTGCTGCCA 50.0 45.0 58.1 58.0 1525-1538 bp 1.0 80.4