Hotspot

ycf1

SSC · 128894-134269 · 5376 bp

Back to Saraca

Pi 0.00447
MarkerSeek score 41.40
Primer available yes
Label ycf1

Feature Metadata

label_name
ycf1
feature_type
gene
region
SSC
length_bp
5376
variable_sites
24
pi
0.00447
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
41.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf1_p1 TTTGCTCCTCTTCCTTCTGC AACTCTTTCAAGCAAACGCG 50.0 45.0 57.8 57.9 192 bp 1.0 79.3
ycf1_p2 ATGCTATCCCAGGTTTCTGC AACTCTTTCAAGCAAACGCG 50.0 45.0 57.6 57.9 256 bp 1.0 77.6
ycf1_p3 TTTGCTCCTCTTCCTTCTGC CTCTACGCAGTCAAAGACGT 50.0 50.0 57.8 57.7 228 bp 1.0 77.2
ycf1_p4 ATGCTATCCCAGGTTTCTGC CTCTACGCAGTCAAAGACGT 50.0 50.0 57.6 57.7 292 bp 1.0 75.6
ycf1_p5 TTTGCTCCTCTTCCTTCTGC TCTCTACGCAGTCAAAGACG 50.0 50.0 57.8 57.4 229 bp 1.0 75.0