Hotspot

petL-petG

LSC · 69074-69259 · 186 bp

Back to Saraca

Pi 0.0216
MarkerSeek score 65.40
Primer available yes
Label petL-petG

Feature Metadata

label_name
petL-petG
feature_type
igs
region
LSC
length_bp
186
variable_sites
4
pi
0.0216
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
65.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petL-petG_p1 ATTGGGCTGAGCAAGATACG CAACTGATCATCACGTCGGT 50.0 50.0 58.0 58.0 320-321 bp 1.0 80.9
petL-petG_p2 GGCTGAGCAAGATACGACTT CAACTGATCATCACGTCGGT 50.0 50.0 57.7 58.0 316-317 bp 1.0 78.8
petL-petG_p3 ATTGGGCTGAGCAAGATACG TCCAACTGATCATCACGTCG 50.0 50.0 58.0 57.7 322-323 bp 1.0 78.7
petL-petG_p4 ATTGGGCTGAGCAAGATACG GGTCCAACTGATCATCACGT 50.0 50.0 58.0 57.6 324-325 bp 1.0 77.8
petL-petG_p5 GGCTGAGCAAGATACGACTT TCCAACTGATCATCACGTCG 50.0 50.0 57.7 57.7 318-319 bp 1.0 76.6