Hotspot

petA-psbJ

LSC · 66000-66812 · 813 bp

Back to Saraca

Pi 0.0149
MarkerSeek score 68.10
Primer available yes
Label petA-psbJ

Feature Metadata

label_name
petA-psbJ
feature_type
igs
region
LSC
length_bp
813
variable_sites
12
pi
0.0149
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
68.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petA-psbJ_p1 CGTGTCCAAGGTCTCTTGTT TCCGGATTAGGTTCATCCCT 50.0 50.0 57.8 57.5 946-948 bp 1.0 75.8
petA-psbJ_p2 CGTGTCCAAGGTCTCTTGTT CCGGATTAGGTTCATCCCTG 50.0 55.0 57.8 57.4 945-947 bp 1.0 75.3
petA-psbJ_p3 CGTTACGTGTCCAAGGTCTC TCCGGATTAGGTTCATCCCT 55.0 50.0 58.3 57.5 951-953 bp 1.0 75.2
petA-psbJ_p4 GATCCGTTACGTGTCCAAGG TCCGGATTAGGTTCATCCCT 55.0 50.0 58.4 57.5 955-957 bp 1.0 74.8
petA-psbJ_p5 CGTTACGTGTCCAAGGTCTC CCGGATTAGGTTCATCCCTG 55.0 55.0 58.3 57.4 950-952 bp 1.0 74.7