Hotspot

ycf4-cemA

LSC · 63708-64131 · 424 bp

Pi 0.00783

MarkerSeek score 63.00

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 424
variable_sites: 3
pi: 0.00783
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 63.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	GAACAAAAAGCGGCGGAATT	GAGATCCACCAGGGCAAAAA	45.0	50.0	58.2	58.1	511-552 bp	1.0	82.1
ycf4-cemA_p2	AACAAAAAGCGGCGGAATTG	GAGATCCACCAGGGCAAAAA	45.0	50.0	58.5	58.1	510-551 bp	1.0	79.8
ycf4-cemA_p3	TGCGTGTACCAATTGAAGGA	GAGATCCACCAGGGCAAAAA	45.0	50.0	57.4	58.1	480-521 bp	1.0	79.4
ycf4-cemA_p4	GAACAAAAAGCGGCGGAATT	AGATCCACCAGGGCAAAAAT	45.0	45.0	58.2	57.4	510-551 bp	1.0	77.6
ycf4-cemA_p5	GACTCCACGAGAAATGGAACA	GAGATCCACCAGGGCAAAAA	47.6	50.0	58.0	58.1	527-568 bp	1.0	75.5