Hotspot

accD-psaI

LSC · 62243-62845 · 603 bp

Back to Saraca

Pi 0.00342
MarkerSeek score 63.80
Primer available yes
Label accD-psaI

Feature Metadata

label_name
accD-psaI
feature_type
igs
region
LSC
length_bp
603
variable_sites
2
pi
0.00342
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.97
markerseek_score
63.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
accD-psaI_p1 TCAATCGTACCGCGTAATCC CCATTGCAATTGCCGGAAAT 50.0 45.0 57.9 58.0 753-769 bp 1.0 81.0
accD-psaI_p2 CAATCGTACCGCGTAATCCT CCATTGCAATTGCCGGAAAT 50.0 45.0 57.9 58.0 752-768 bp 1.0 81.0
accD-psaI_p3 AGGGCGTTCTGAGTGAGTTA CCATTGCAATTGCCGGAAAT 50.0 45.0 58.4 58.0 728-744 bp 1.0 79.1
accD-psaI_p4 TCAATCGTACCGCGTAATCC AACGAAGCCATTGCAATTGC 50.0 45.0 57.9 58.6 760-776 bp 1.0 76.6
accD-psaI_p5 CAATCGTACCGCGTAATCCT AACGAAGCCATTGCAATTGC 50.0 45.0 57.9 58.6 759-775 bp 1.0 76.6