Hotspot

ndhC-trnV-UAC

LSC · 54022-54600 · 579 bp

Back to Saraca

Pi 0.0112
MarkerSeek score 68.90
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
579
variable_sites
6
pi
0.0112
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.93
markerseek_score
68.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 TGCCAAAATGGGAATAACGC AGAAGGTCTACGGTTCGAGT 45.0 50.0 57.3 57.8 684-713 bp 1.0 77.8
ndhC-trnV-UAC_p2 CTCTGGCCCTTTGCTAATCG AGAAGGTCTACGGTTCGAGT 55.0 50.0 58.7 57.8 729-758 bp 1.0 77.5
ndhC-trnV-UAC_p3 TCTCTGGCCCTTTGCTAATC AGAAGGTCTACGGTTCGAGT 50.0 50.0 57.3 57.8 730-759 bp 1.0 77.3
ndhC-trnV-UAC_p4 GCCAAAATGGGAATAACGCT AGAAGGTCTACGGTTCGAGT 45.0 50.0 57.0 57.8 683-712 bp 1.0 75.4
ndhC-trnV-UAC_p5 TTTCTCTGGCCCTTTGCTAA AGAAGGTCTACGGTTCGAGT 45.0 50.0 57.0 57.8 732-761 bp 1.0 75.3