Hotspot

trnT-GGU-psbD

LSC · 34126-35509 · 1384 bp

Back to Saraca

Pi 0.00509
MarkerSeek score 62.40
Primer available yes
Label trnT-GGU-psbD

Feature Metadata

label_name
trnT-GGU-psbD
feature_type
igs
region
LSC
length_bp
1384
variable_sites
7
pi
0.00509
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
62.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-GGU-psbD_p1 GTGGTAGAGTAACGCCATGG ACAAAAACGAAACGGTCCCT 55.0 45.0 58.1 58.2 1524-1534 bp 1.0 78.4
trnT-GGU-psbD_p2 GTGGTAGAGTAACGCCATGG GACCGGACCAACCTACAAAA 55.0 50.0 58.1 57.7 1538-1548 bp 1.0 78.3
trnT-GGU-psbD_p3 TCAGTGGTAGAGTAACGCCA ACAAAAACGAAACGGTCCCT 50.0 45.0 58.1 58.2 1527-1537 bp 1.0 78.2
trnT-GGU-psbD_p4 TCAGTGGTAGAGTAACGCCA GACCGGACCAACCTACAAAA 50.0 50.0 58.1 57.7 1541-1551 bp 1.0 78.1
trnT-GGU-psbD_p5 GTGGTAGAGTAACGCCATGG TAGACCGGACCAACCTACAA 55.0 50.0 58.1 57.7 1540-1550 bp 1.0 77.9