Hotspot

psbM-trnD-GUC

LSC · 31503-32416 · 914 bp

Back to Saraca

Pi 0.00158
MarkerSeek score 49.40
Primer available yes
Label psbM-trnD-GUC

Feature Metadata

label_name
psbM-trnD-GUC
feature_type
igs
region
LSC
length_bp
914
variable_sites
1
pi
0.00158
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.69
markerseek_score
49.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbM-trnD-GUC_p1 AGAATGAACAGTGCAGTAGC TCCCTGGGACTGTAGTTCAA 45.0 50.0 55.8 57.6 757-1040 bp 1.0 63.0
psbM-trnD-GUC_p2 AGAATGAACAGTGCAGTAGC TTCCCTGGGACTGTAGTTCA 45.0 50.0 55.8 57.6 758-1041 bp 1.0 63.0
psbM-trnD-GUC_p3 AGAATGAACAGTGCAGTAGC TTCAATTGGTCAGAGCACCG 45.0 50.0 55.8 58.5 742-1025 bp 1.0 62.4
psbM-trnD-GUC_p4 AGGAACTAGAATGAACAGTGCA TCCCTGGGACTGTAGTTCAA 40.9 50.0 57.4 57.6 764-1047 bp 1.0 59.8
psbM-trnD-GUC_p5 AGGAACTAGAATGAACAGTGCA TTCCCTGGGACTGTAGTTCA 40.9 50.0 57.4 57.6 765-1048 bp 1.0 59.8