Hotspot

trnR-UCU-atpA

LSC · 10414-10844 · 431 bp

Back to Saraca

Pi 0.00957
MarkerSeek score 50.50
Primer available yes
Label trnR-UCU-atpA

Feature Metadata

label_name
trnR-UCU-atpA
feature_type
igs
region
LSC
length_bp
431
variable_sites
4
pi
0.00957
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.97
markerseek_score
50.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnR-UCU-atpA_p1 GGTTCAAATCCTATTGGACGC TCAGGAACAAGTCGAAAAA 47.6 36.8 57.3 52.3 478-507 bp 1.0 42.8
trnR-UCU-atpA_p2 GTTCAAATCCTATTGGACGCA TCAGGAACAAGTCGAAAAA 42.9 36.8 56.6 52.3 477-506 bp 1.0 42.8
trnR-UCU-atpA_p3 TGGATAGGACAGAGGTCTTCT TCAGGAACAAGTCGAAAAA 47.6 36.8 56.9 52.3 513-542 bp 1.0 42.6
trnR-UCU-atpA_p4 ATGGATAGGACAGAGGTCTTCT TCAGGAACAAGTCGAAAAA 45.5 36.8 57.4 52.3 514-543 bp 1.0 42.6
trnR-UCU-atpA_p5 AGGACAGAGGTCTTCTAAACC TCAGGAACAAGTCGAAAAA 47.6 36.8 56.4 52.3 508-537 bp 1.0 42.6