Hotspot

trnS-GCU-trnG-UCC

LSC · 8458-9302 · 845 bp

Back to Saraca

Pi 0.00986
MarkerSeek score 54.60
Primer available yes
Label trnS-GCU-trnG-UCC

Feature Metadata

label_name
trnS-GCU-trnG-UCC
feature_type
igs
region
LSC
length_bp
845
variable_sites
8
pi
0.00986
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.96
markerseek_score
54.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-UCC_p1 CCACTCAGCCATCTCTCC AACGAATCGCCCTTTTACCA 61.1 45.0 56.4 57.8 891-898 bp 1.0 53.5
trnS-GCU-trnG-UCC_p2 CCACTCAGCCATCTCTCC CGAATCGCCCTTTTACCACT 61.1 50.0 56.4 58.3 889-896 bp 1.0 52.9
trnS-GCU-trnG-UCC_p3 CCACTCAGCCATCTCTCC ATGAATCAAACGAGGGACCC 61.1 50.0 56.4 57.6 934-941 bp 1.0 51.4
trnS-GCU-trnG-UCC_p4 CCACTCAGCCATCTCTCC ACGAATCGCCCTTTTACCAC 61.1 50.0 56.4 58.6 890-897 bp 1.0 50.5
trnS-GCU-trnG-UCC_p5 CCACTCAGCCATCTCTCC GAACGAATCGCCCTTTTACC 61.1 50.0 56.4 57.2 892-899 bp 1.0 48.4