Hotspot

ycf1

SSC · 127201-132840 · 5640 bp

Back to Sapindus

Pi 0.00118

MarkerSeek score 53.30

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5640
variable_sites: 10
pi: 0.00118
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TGGCAGTGATGAATTTGTCCA	TGGAGCATCGAAAGCATTCT	42.9	45.0	58.1	57.6	207 bp	1.0	68.9
ycf1_p2	GGCAGTGATGAATTTGTCCAC	TGGAGCATCGAAAGCATTCT	47.6	45.0	57.8	57.6	206 bp	1.0	68.1
ycf1_p3	CTGGCAGTGATGAATTTGTCC	TGGAGCATCGAAAGCATTCT	47.6	45.0	57.5	57.6	208 bp	1.0	65.9
ycf1_p4	GGCAGTGATGAATTTGTCCA	TGGAGCATCGAAAGCATTCT	45.0	45.0	56.3	57.6	206 bp	1.0	64.4
ycf1_p5	TGGCAGTGATGAATTTGTCC	TGGAGCATCGAAAGCATTCT	45.0	45.0	56.3	57.6	207 bp	1.0	64.4