Hotspot

rpoC2

LSC · 17294-21418 · 4125 bp

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Pi 0.00129

MarkerSeek score 44.90

Primer available yes

Label rpoC2

Feature Metadata

label_name: rpoC2
feature_type: gene
region: LSC
length_bp: 4125
variable_sites: 8
pi: 0.00129
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 44.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC2_p1	TCGAGATACCTGAATGGGGC	AATCATCAAGTGCGACACCA	55.0	45.0	59.0	57.8	241 bp	1.0	71.6
rpoC2_p2	TCGAGATACCTGAATGGGGC	ATCATCAAGTGCGACACCAA	55.0	45.0	59.0	57.8	240 bp	1.0	71.6
rpoC2_p3	TCGAGATACCTGAATGGGGC	TCATCAAGTGCGACACCAAT	55.0	45.0	59.0	57.8	239 bp	1.0	71.6
rpoC2_p4	CGAGATACCTGAATGGGGCA	ATCATCAAGTGCGACACCAA	55.0	45.0	59.2	57.8	239 bp	1.0	69.3
rpoC2_p5	CGAGATACCTGAATGGGGCA	TCATCAAGTGCGACACCAAT	55.0	45.0	59.2	57.8	238 bp	1.0	69.3