Hotspot

accD

LSC · 59478-60968 · 1491 bp

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Pi 0.00134

MarkerSeek score 59.60

Primer available yes

Label accD

Feature Metadata

label_name: accD
feature_type: gene
region: LSC
length_bp: 1491
variable_sites: 3
pi: 0.00134
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD_p1	GTGAAGCAATATTCCGGGGT	TAACTAACCAGCTGTTCGCC	50.0	50.0	57.9	57.9	1632 bp	1.0	79.0
accD_p2	TGAAGCAATATTCCGGGGTC	TAACTAACCAGCTGTTCGCC	50.0	50.0	57.7	57.9	1631 bp	1.0	76.7
accD_p3	AGTGAAGCAATATTCCGGGG	TAACTAACCAGCTGTTCGCC	50.0	50.0	57.7	57.9	1633 bp	1.0	76.6
accD_p4	AAGCAATATTCCGGGGTCTC	TAACTAACCAGCTGTTCGCC	50.0	50.0	57.4	57.9	1629 bp	1.0	74.3
accD_p5	GAAGCAATATTCCGGGGTCT	TAACTAACCAGCTGTTCGCC	50.0	50.0	57.4	57.9	1630 bp	1.0	74.3